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Although many protein labeling probes have been developed to elucidate the trafficking and turnover processes of cell surface proteins, real-time tracking of intracellular proteins remains a challenging task. Herein, we describe a new design to construct a cell-permeable, photostable, and far-red fluorescent turn-on probe to enable no-wash, organelle-specific, and long-term visualization of intracellular SNAP-tagged proteins in living cells. When the probe was used in dual-color pulse chase labeling experiments to differentiate between preLamin and mature Lamin, our results reveal that the shape of mature Lamin can be altered by the newly synthesized preLamin and that this alteration is progressive, cumulative, and due to a concentration-dependent dominant-negative effect of preLamin. We believe that this probe can also be applied to other intracellular proteins whose cellular localization and synthesis changes dynamically in response to external stimuli.
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Given how little sex or sensuality there is in mainstream American cinema these days, it's no surprise that the Magic Mike movies have been so popular. The first Magic Mike, directed by Steven Soderbergh in 2012, was an irresistible showcase for Channing Tatum and his thong-and-dance routine, though it was also a sharp, realistic portrait of cash-strapped workers getting by in post-recession Florida. Three years later, the director Gregory Jacobs leaned into the erotic spectacle of it all with the exuberant Magic Mike XXL, placing women's desires front and center in a way that made even the first movie look staid.
The possibility of long-term romance didn't really factor into the first two Magic Mike movies, which were all about fleeting transactional encounters. I guess that makes Magic Mike's Last Dance the more mature, thoughtful entertainment, and I'm not sure that's entirely a good thing. Don't get me wrong: Tatum and Hayek Pinault have an on-screen chemistry that's both romantic and collaborative. Their characters' creative back-and-forth becomes a vision of gender parity in action: Max wants to thrill her play's female audience, but she needs Mike's smarts and expertise to do it. Still, there's something a little too dutiful and even dull about the way the characters' mutual attraction ultimately plays out. 041b061a72